Conidial and mycelial-bound exo-pectinase of Aspergillus sp.
نویسندگان
چکیده
منابع مشابه
The weak acid preservative sorbic acid inhibits conidial germination and mycelial growth of Aspergillus niger through intracellular acidification.
The growth of the filamentous fungus Aspergillus niger, a common food spoilage organism, is inhibited by the weak acid preservative sorbic acid (trans-trans-2,4-hexadienoic acid). Conidia inoculated at 10(5)/ml of medium showed a sorbic acid MIC of 4.5 mM at pH 4.0, whereas the MIC for the amount of mycelia at 24 h developed from the same spore inoculum was threefold lower. The MIC for conidia ...
متن کاملProduction kinetics and characterization of pectinase enzyme from Aspergillus niger
Food grade pectinases from Aspergillus niger are predominantly used as processing aids in industries. In the present investigation, efforts towards standardization for pilot scale production of pectinase enzyme from A. niger were made. The crude enzyme was studied for its kinetics to optimize substrate concentration and processing parameters on the basis of polygalacturonic activity. Km (Michae...
متن کاملConidial viability assay for rapid susceptibility testing of Aspergillus species.
Antifungal susceptibility testing of filamentous fungi has become more important given the recognition of drug-resistant organisms and the availability of therapies other than amphotericin B (AMB). As current microdilution and E-test methods are limited by a 2 to 3 day incubation time required to obtain results, a more rapid method for susceptibility testing of fungi is needed. We report here a...
متن کاملExploitation of Fruit Wastes for Pectinase Production Using Aspergillus Oryzae
Ever increasing population and industrialization has resulted in heavy pollution and waste generation. The main aim of this project lies in waste management and synthesis of pectinase enzyme which plays a crucial role in sewage treatment. Twenty moulds were isolated from decaying fruits and municipal waste soil sample on SDA and were further screened for pectinolytic enzyme production. Screenin...
متن کاملAffinity precipitation of Aspergillus niger pectinase by microwave-treated alginate.
Affinity precipitation is a simple, single plate separation process in which the complex of a smart macroaffinity ligand with the target protein (from a crude broth) can be selectively precipitated by application of a suitable stimulus. Alginate is a copolymer of guluronic acid and mannuronic acid residues and precipitates with Ca(2+) ions. It was found to bind to pectinase present in a commerc...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1993
ISSN: 0378-1097
DOI: 10.1016/0378-1097(93)90573-k